Feline Blood Typing Rapid Test Cassette
(Whole Blood)
REF VIFAB-402
A rapid test for the qualitative detection of Feline Blood Typing A and B and AB antigen in feline whole blood specimen.
INTENDED USE
The Feline Blood Typing Rapid Test Cassette (Whole Blood) is a solid-phase method test for determining the regular feline AB blood
typing.
PRINCIPLE
The assay starts with a sample applied to the specimen well and add provided washing buffer later to the “Buffer” well. The monoclonal
antibody immobilized respectively on the pad in sample region can react with the corresponding antigen on the surface of the red blood
cells (RBC). A positive sample produces an immune response and RBC can be captured on the sample region as a red signal, indicating that the test is positive. A negative sample does not produce an immune response, after adding washing buffer, the RBC can be washed away, and the absence of the RBC indicates that the test is negative.
MATERIALS
Materials Provided
• Test Cassettes
• Droppers
• Washing Buffer
• Package Insert
Materials Required But Not Provided
• Timer
• Pipette
• Scissors
PRECAUTIONS
Please read all the information in this package insert before performing the test.
1.For veterinary diagnostic use only. Do not use after the expiration date.
2.The test should remain in the sealed pouch until ready to use.
3.All specimens should be considered potentially hazardous and handled in the same manner as an infectious agent.
4.The used test should be discarded according to the local regulations.
5.Please make sure that an appropriate amount of samples are used for testing. Too much or too little sample volume may lead to result deviation.
6.As this product is visual inspection, please do not interpret in dim light to ensure accurate results.
STORAGE AND STABILITY
Store as packaged at room temperature or refrigerated (2-30 °C). The test is stable through the expiration date printed on the sealed pouch. The test must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.
DIRECTIONS FOR USE
Allow the test, specimen and buffer to reach room temperature
(15-30 °C) prior to testing.
1. Remove the test cassette from the sealed foil pouch and use it as soon as possible. Best results will be obtained if the assay is
performed within one hour.
2. Place the cassette on a clean and level surface.
3. Hold the dropper vertically and transfer 1 drop of whole blood (approximately 10 L) to “A”, “B”, “C” well of test cassette separately. Let it stands for 3-5 minutes. “C” well is for quality control.
4. Before use the buffer, make sure that the buffer is left at the bottom of the buffer bottle. If it is at the neck of the bottle, shake it firmly
several times to return the buffer to its place. Cut the bottleneck of the buffer with scissors and add 2 vials of washing buffer to “Buffer” well.
5. Interpret test results at 2 minutes on “A” “B” “C” well. Do not interpret the result after 15 minutes.
