TRI Antibody Rapid Test Cassette (Whole Blood/Serum/Plasma), detection of antibodies to Trichinella
| Name: |
TRI Antibody Rapid Test Cassette (Whole Blood/Serum/Plasma), Detection Of Antibodies To Trichinella |
| Principle: |
Chromatographic Immunoassay |
| Specimen: |
Whole Blood/Serum/Plasma |
| Pack: |
10 T |
| Shelf Life: |
2 Years |
| Specificity: |
92.45% |
| Cat NO.: |
VITR-402 |
| Format: |
Cassette |
| Reading Time: |
5-10 Minutes |
| Storage Temperature: |
2-30°C |
| Sensitivity: |
89.36% |
| Accuracy: |
91.00% |
INTENDED USE
The TRI Antibody Rapid Test Cassette (Whole Blood/ Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of antibodies to Trichinella in swine’s whole blood or serum and plasma.
PRINCIPLE
The TRI Antibody Rapid Test Cassette (Whole Blood/ Serum/Plasma) is a qualitative, membrane based immunoassay with a double antigen system for the detection of Trichinella antibodies in serum or plasma. The membrane is pre-coated with recombinant TRI antigens. The test cassette has a testing window. The testing window has an invisible T (test) line region and C (control) line region.
During testing, antibodies to TRI, if present in whole blood, serum or plasma specimen react with TRI antigen coated colloidal gold particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and
reacts with recombinant TRI antigens on the membrane in the test line region. If the specimen contains antibodies to trichinella, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain trichinella antibodies, a colored line will not appear in the test line region indicating a negative result.
To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test cassette is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use.
DO NOT FREEZE. Do not use beyond the expiration date
DIRECTIONS FOR USE
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to
testing.
1. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Collect swine’s fresh whole blood or separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C.
3. Place the test cassette on a clean and level surface.
4. For serum and plasma: Hold the dropper vertically and draw the specimen up to the Fill Line (approximately 5 µL), and transfer the specimen to the specimen well (S) of the test cassette, wait for 8-10 seconds, then add 2 drops of buffer (approximately 80 µl) and start the timer.
For whole blood: Hold the dropper vertically, draw the specimen above 1cm above the Fill Line, and transfer 1 drop of whole blood (approximately 10 µL) to the specimen well (S) of the test cassette, wait for 8-10 seconds, then add 2 drops of buffer (approximately 80 µL) and start the timer.
5. Read the result in 5-10 minutes. Do not interpret results after 15 minutes.
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, regardless of T line being strong or faint.
Negative: Only clear C line appears.
Invalid: No colored line appears in C line region, regardless of T line’s appearance.
