Medical Home Dengue NS1 Antigen Test Kit , Dengue NS1 Ag Rapid Test Kit

Dengue NS1 Test Cassette Rapid Medical Home, Fluorescence Immunoassay Test

The Dengue NS1 Test Cassette is a rapid fluorescence immunoassay for the qualitative detection of NS1 antigen of Dengue virus in human whole blood, serum, or plasma as an aid in the diagnosis of Dengue infections.The test result is calculated by Fluorescence Immunoassay.​

Applications:

The Dengue IgG/IgM Ab Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative membrane-based immunoassay for the detection of Dengue antibodies in whole blood, serum, or plasma.

The Dengue IgG/IgM Test Cassette is based on Fluorescence Immunoassay for qualitative detection of Dengue IgG and IgM antibodies in human whole blood, serum, or plasma. It is intended to aid in the rapid diagnosis of Dengue infections.The test result is calculated by Fluorescence Immunoassay Analyzer.

Description:  

The Dengue IgG/IgM Ab Rapid Test Cassette (Whole Blood / Serum/ Plasma) is a qualitative membrane-based immunoassay for the detection of Dengue antibodies in whole blood, serum, or plasma. This test consists of two components, an IgG component and an IgM component. In the IgG component, anti-human IgG is coated in IgG test line region. During testing, the specimen reacts with Dengue antigen coated particles in the test cassette. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the anti-human IgG in IgG test line region. If the specimen contains IgG antibodies to Dengue, a colored line will appear in IgG test line region. In the IgM component, anti-human IgM is coated in IgM test line region. During testing, the specimen reacts with antihuman IgM. Dengue IgM antibodies, if present in the  specimen, reacts with the antihuman IgM and the Dengue antigen-coated  particles in the test cassette, and this complex is captured by the anti-human IgM, forming a colored line in IgM test line region.

Therefore, if the specimen contains Dengue IgG antibodies, a colored line will appear in t IgG test line region. If the specimen contains Dengue IgM antibodies, a colored line will appear in IgM test line region. If the specimen does not contain Dengue antibodies, no colored line will appear in either of the test line regions, indicating a negative result.

How to use? 

Allow the test cassette, specimen, buffer, and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface.
· For Serum or Plasma Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 5 μl), and transfer the specimen to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40ul) and start the timer. Avoid trapping air bubbles in the specimen well.
To use a micropipette: Pipette and dispense 5 µl of specimen to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.
· For Whole Blood (Venipuncture/Fingerstick) Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen about 1cm above the Fill Line,
and transfer 1 drop of whole blood (approximately 10 µl) to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40 ul) and start the timer.
To use a micropipette: Pipette and dispense 10 µl of whole blood to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.

3. Wait for the colored line(s) to appear. The test result should be read at 10 minutes. Do not interpret the result after 20 minutes.

INTERPRETATION OF RESULTS 

(Please refer to the illustration above)

IgG and IgM POSITIVE:* Three lines appear. One colored line should be in the control line region (C), and two colored lines should appear in IgG test line region and IgM test line region. The color intensities of the lines do not have to match. The result is positive for IgG & IgM antibodies indicated end stage of primary Dengue infection and early stage of secondary Dengue infection .

IgG POSITIVE:* Two lines appear.One colored line should be in the control line region (C), and a colored line appears in IgG test line region. The result is positive for Dengue virus specific-IgG and is probably indicative of secondary Dengue infection.
IgM POSITIVE:* Two lines appear.One colored line should be in the control line region (C), and a colored line appears in IgM test line region. The result is positive for Dengue virus specific-IgM antibodies and is indicative of primary Dengue infection.
*NOTE: The intensity of the color in the IgG and/or IgM test line region(s) will vary depending on the concentration of Dengue antibodies in the specimen. Therefore, any shade of color in the IgG and/or
IgM test line region(s) should be considered positive.
NEGATIVE: One colored line should be in the control line region (C). No line appears in IgG and IgM test line region(s).

INVALID: Control line fails to appear. Insufficient buffer volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the procedure with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.